The factors that affect enzyme catalysis (that is, enzyme activity) will definitely affect the rate of enzymatic reactions, but the factors that affect the rate of enzymatic reactions do not necessarily affect the catalysis. pH, temperature, ultraviolet light, heavy metal salts, inhibitors, activators, etc. will affect the catalysis and the rate of enzymatic reactions. The concentration of the enzyme, the concentration of the substrate, etc. will not affect the enzyme catalysis, but can affect the rate of the enzymatic reaction. Factors affecting enzyme catalysis are:

1. Effect of temperature on enzyme activity

Temperature has a great influence on the speed…


The appearance of pectinase is light yellow powder, and it is called one of the four major enzyme preparations. In fact, pectinase does not specifically refer to a certain enzyme, but a general term for a class of enzymes that decompose pectin, including galacturonidase, pectin-decomposing enzyme and pectin lipase.

Pectinase can decompose pectin, disintegrate plant cell walls and intercellular layers, making it easier to squeeze juice, and decompose pectin into soluble galacturonic acid, which will also make cloudy juice become clear. Pectinase is mainly found in higher plants and microorganisms, and it is also found in some protozoa and insects…


The International System Classification of Enzymes proposed by the Enzymology Committee of the International Society of Biochemistry divides all known enzymes into six categories according to the types of reactions they catalyze, which are represented by numbers 1, 2, 3, 4, 5, and 6, respectively. Next, let’s take a closer look at the enzyme classification of these six different enzymes.

1. Oxidoreductases

All enzymes that can catalyze the oxidation-reduction reaction of a substrate are called oxidoreductases. In organic reactions, dehydrogenation and oxygenation are usually regarded as oxidation, and hydrodeoxygenation is regarded as reduction. Such enzymes include dehydrogenases, oxygenases (oxygenases), oxidases…


There are two types of enzymes produced by biological cells: one type of enzymes that are produced inside the cell and then secreted outside the cell for action, called extracellular enzymes. Most of these enzymes are hydrolases. For example, the two amylases used in enzymatic production of glucose are secreted by Bacillus subtilis and root enzymes during fermentation. Such enzymes are generally high in content and easy to obtain.

Another type of enzyme is produced in the cell and is not secreted outside the cell, but plays a catalytic role in the cell, which is called intracellular enzyme. A series…


Ligase is an enzyme that catalyzes the joining of two molecules into one molecule or the end to end of a molecule. This reaction is coupled with the decomposition reaction of ATP. When the two molecules are connected, the high-energy phosphate bond of adenosine triphosphate (ATP) is broken, such as DNA ligase. Ligase is an important class in enzyme classification (EC 6): 6.1 forms C-O bonds; 6.2 forms C-S bonds; 6.3 forms C-N bonds; 6.4 forms C-C bonds.

DNA ligase was first discovered at the same time by three laboratories in 1967. After decades of research by scientists, it has…


As a substance, catalase was first discovered in 1811 by Louis Jacques Thénard, the discoverer of hydrogen peroxide. In 1900, Oscar Loew named this enzyme capable of degrading hydrogen peroxide “catalase”, and discovered that this enzyme exists in many plants and animals. In 1937, James B. Sumner crystallized catalase from bovine liver and obtained the molecular weight of the enzyme the following year. In 1969, the amino acid sequence of cattle catalase was solved. Then, in 1981, its three-dimensional structure was resolved.

Catalase exists in almost all living organisms. It is commonly found in breathing organisms, mainly in plant chloroplasts…


In 2021, the Coronavirus Pandemic continues to affect the work and lives of people all over the world. In such a dark moment, the world is experiencing a severe economic recession and a decline in global trade volume. Countries in the world must first ensure food safety and food reserve security. Under such a scenario, what impact will it have on the nutrition and health industry? What is the future development trend of the industry? And what are the uncertain fluctuations?

1. Strong growth in the food supplement industry during the epidemic

Many industries have fallen into a long-term downturn…


Ascorbate oxidase is a copper-containing enzyme, located in the cytoplasm or combined with the cell wall, and coupled with other redox reactions to act as a terminal oxidase. It can catalyze the oxidation of ascorbic acid, has anti-aging effects, and plays an important role in material metabolism in plants. Under the catalysis of this enzyme, molecular oxygen can oxidize ascorbic acid to dehydroascorbic acid, which exists in the cytosol and cell wall, and the enzyme contains copper. Pyruvate, isocitrate, a. Ketoglutarate, malic acid, glucose, 6. Phosphoric acid, 6. Phosphogluconic acid can dehydrogenate protons under the action of dehydrogenase. The hydrogen…


Helicobacter pylori (Hp) is closely related to the occurrence and development of gastric cancer, peptic ulcer, Hp-related dyspepsia and other diseases. Studies have found that Hp can cause gastric microbial disorders, change the microbial diversity and community structure in the stomach, and cause precancerous lesions. The public health community has made great efforts to eradicate Hp for a long time, but the current Hp infection rate in the entire population is still over 50%. In recent years, probiotics have attracted attention due to their relative safety, and microecological therapy has brought new ideas for the treatment of Hp.

Probiotics are…


Probiotics refer to a certain number of live microorganisms that can have a beneficial effect on the health of the host. At present, there are more than 20 kinds of probiotics used in China, mainly including Bifidobacterium, Lactobacillus, Clostridium butyricum, Saccharomyces boulardii, Enterococcus, Bacillus licheniformis and Bacillus cereus. Probiotics have eight major functions.

1. Maintain normal intestinal function

The intestine is the human body’s largest immune organ and the largest micro-ecological system. The human body interacts slowly with the outside world through the mucosa of the gastrointestinal tract. …

Iva Colter

Creative Enzymes is a remarkable supplier and manufacturer in the Enzymology field.

Get the Medium app

A button that says 'Download on the App Store', and if clicked it will lead you to the iOS App store
A button that says 'Get it on, Google Play', and if clicked it will lead you to the Google Play store